Departs from the Moonee Ponds Junction bus terminal and runs via Mt. Alexander Road, Buckley Street, Russell Street to Essendon Railway Station. Then via Russell Street, Napier Street, Hayes Road, Carnarvon Road, York Street, Napier Street, Loch Crescent, Fenacre Street, Lebanon Street, Eldorado Street, Streldon Avenue, Woolart Street, Mascoma Street, Lockheed Street, Boeing Road, Mascoma Street, Matthews Avenue, Dromana Avenue to Westfield Shoppingtown. Then via Louis Street, Moore Road, Louis Street, Marshall Road, Sexton Street, Parer Road, Etzel Street, Clydesdale Road, South Road, McNamara Avenue, Roberts Road, El Reno Crescent, Grange Road, Fullarton Road, Matthews Avenue to the terminus at the corner of Matthews Avenue and Keilor Road.

Route 501 from Niddrie to Moonee Ponds

Departs from the terminus at the corner of Matthews Avenue and Keilor Road and runs via Keilor and Grange Roads, then via the reverse of the route described above to the Moonee Ponds Junction bus terminal.

• Times marked "A" indicate bus deviates via Strathmore Secondary College on school days only.
• Times marked "B" indicate bus deviates via St. Vincents Primary School on school days only.
• Times marked "C" indicate bus deviates via Essendon and Keilor College (Niddrie Campus) on school days only.
• There is no service on Sundays or Public Holidays.
• A special Monday to Friday timetable operates during the Christmas holiday period.
• Times are subject to prevailing traffic conditions.
• Times may be subject to alteration without notice.
• Train times shown are the designated bus/train connections.