Departs from the Moonee Ponds Junction bus terminal and runs via Mt Alexander Road, Buckley Street, Russell Street to Essendon Railway Station. Then via Russell Street, Mt Alexander Road, Shamrock Street, Richardson Street, Thistle Street, Florence Street, Lincoln Road, Woolley Street, Hedderwick Street, Mary Street, Hoffmans Road, Rosehill Road, Garnet Street, Vaynor Street, Hoffmans Road, Keilor Road, Newman Street, Hotham Rd, The Avenue, Keilor Road, Woorite Place, Milleara Road, Clarks Road, Quinn Grove, Noga Avenue, Rachelle Road, Dinah Parade, Phillip Road, Buckley Street to the terminus at the corner of Milleara Road and Buckley Street.

Route 475 from East Keilor to Moonee Ponds:

Departs from the terminus at the corner of Milleara Road and Buckley Street and runs via Milleara Road, Woorite Place, then via the reverse of the route described above to Shamrock Street, then via Shamrock and Rose Streets to Essendon Railway Station. Then via Rose Street, Buckley Street, Mt Alexander Road to the Moonee Ponds Junction bus terminal.