Departs from the terminus in Sydney Road (West side), immediately north of Bell Street and runs via Sydney Road, Champ Street, Murray Road, Elizabeth Street, Claremont Street, Jackson Parade, Boyne Street, Elizabeth Street, Hornton Street, Corben Street and Kennedy Street to the terminus in Kennedy Street (South side) immediately east of Elizabeth Street.

ROUTE 526 from West Preston to Coburg:

Departs from the terminus in Kennedy Street and runs via Kennedy Street, Elizabeth Street, Boyne Street then via the reverse of the route described above to Sydney Road, then via Sydney Road, Wilson Street, McKay Street and Bell Street to the terminus in Sydney Road.

• Times marked in "W" indicate trips that will be operated by ultra low floor, wheelchair accessible buses.
• There is no service on Sundays or Public Holidays.
• Normal Saturday service operates on Easter Saturday.
• Times may be subject to alteration without notice.
• Train times shown are the designated bus/train connections.